Research Journal of Pharmacy and Technology

R. Devika ¹, S. Chozhavendhan ¹, G. Karthigadevi ¹, Shalini Chauhan ¹

Affiliations
1 Department of Biotechnology, Aarupadai Veedu Institute of Technology, Vinayaka Missions Research Foundation (Deemed to be University), Paiyanoor – 603104, IN

Abstract

Over the past few decades, curing of diseases are under threat as many medicines and drugs produce toxic reactions. Nowadays 61% of new drugs has been developed using natural phytochemicals in curing many diseases. Present investigation is an attempt to assess the antimicrobial activity of Pergularia daemia and to evaluate the potential against antibiotics. In the present study, P.daemia proved to have efficient phytochemicals which created distinct zone of inhibition against bacterial strains.

Keywords

Antibacterial, Zone Of Inhibition, Resistance, Susceptible, Phytochemicals.

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R. Devika ¹, Shalini Chauhan ¹, R. Subbaiya ¹

Affiliations
1 Department of Biotechnology, Aarupadai Veedu Institute of Technology, Vinayaka Missions Research Foundation (Deemed to be University), Paiyanoor – 603104, IN

Abstract

Nerium oleander leaves were collected from Sipcot IT Park and the leaf extract was prepared withdeionized water and used for the synthesis of copper nanoparticles. The colour of the solution changes from yellowish green to dark brown, which confirmed the formation of copper nanoparticles. CuNPs were characterized by UV-Vis spectroscopy from which absorbance peak was found at 320-620 nm. CuNPs confirmed natural bio compounds found in the leaf extract by FTIR peaks that are responsible for reducing and stabilizing agent. SEM micrographs of the synthesized by the reduction of copper sulphate revealed spherical and crystalline which ranged in an average size of 20 nm. Biological method found to be eco-friendly, cost effective method, which provided natural capping agent.

Keywords

CuNPs, UV, SEM, FTIR, Absorbance, Extract, Characterization.

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R. Devika ¹, P. Mohanapriya ¹, Rahul Kumar ¹

Affiliations
1 Department of Biotechnology, Aarupadai Veedu Institute of Technology, Vinayaka Missions Research Foundation, Paiyanoor – 603104., IN

Abstract

Bauhinia tomentosa commonly known as yellow bell orchid tree belongs to Fabaceae family and it is best versatile and most commonly used household remedy for many manifestations. The aqueous leaf extract of Bauhinia tomentosa were subjected to produce silver nanomaterials. When aqueous extract added to chemical solution the reduction process occured and the colour change from light green into dark brown was visually observed. The synthesized nanomaterials were characterized by using Ultra Violet Spectrophotometer and FT- IR analysis. The UV spectrophotometer characterization of synthesized nanoparticles at different proportions such as 1:1, 2:2, 3:3, 4:4 and 5:5 of extract and solution mediated synthesized nanoparticles showed a maximum absorbance at the range of 450 to 550nm. FT- IR analysis of silver, copper and iron nanoparticles showed the presence of alcohol/ phenol group (-OH), alkyl stretch (C-H), aromatic (C=C) bending, and amine (C- N) functional groups present was confirmed.

Keywords

Nanoparticles, Plant Extracts, Optimization, Spectrophotometer, FT-IR.

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R. Subbaiya ¹, B. Aakash ¹, A. Shanmugaraja ², R. Devika ¹, S. Chozhavendhan ¹, S. Vinoth ¹, G. Karthiga Devi ¹, M. Masilamani Selvam ³, S. Chozhavendhan ⁴

Affiliations
1 Department of Biotechnology, Aarupadai Veedu Institute of Technology, Vinayaka Missions Research Foundation (Deemed to be University) Paiyanoor, Chennai, IN
2 Department of Biotechnology, K. S. Rangasamy College of Technology, Tiruchengode, Namakkal – 637215 Tamil Nadu, IN
3 Department of Biotechnology, Satyabhama Institute of Science and Technology, Jeppiaar Nagar, Chennai – 600 119, IN
4 Department of Biotechnology, Vivekanandha College of Engineering For Women, Elayampalayam, Tiruchengode, IN

Abstract

The clean production concept shows a good utilization potential for solid vegetable waste. It could achieve a reduction of investment and raw materials and can contribute to waste minimised plant production. Thus rendering cheap and environmentally safe alternative to commercial media. At present study was aimed at finding the potential of vegetable waste as a culture media. Composition of the media and the efficiency of the media to support plant growth were tested. Vegetable waste of Cabbage, Beetischolar_main and Onion were collected and dried in the sun light for 2 weeks. Dried vegetable waste was grinded into fine powder. These vegetable waste media (alternative plant tissue culture media) was prepared and coconut water was added for natural hormone (cytokinin). The alternative media pH was 5.6 to 5.7 before sterilization. The vegetable waste powder was analysed for Macronutrients which include potassium (268 mg/kg), sodium (520 mg/kg), calcium (1591 mg/kg), while the micronutrients were analysed copper (5.421 mg/kg), iron (1101 mg/kg) and zinc. The growth was comparable to that obtained on routine commercial media.

Keywords

Vegetable Waste, BCO, Plant Tissue Culture Media, Cost Effective.

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S. Vinoth ¹, N. Rathika ¹, M. Jhansi ¹, P. Gurusaravanan ², M. Vigneswaran ³, R. Subbaiya ¹, G. Karthiga Devi ¹, S. Chozhavendhan ¹, R. Devika ¹

Affiliations
1 Department of Biotechnology, Aarupadai Veedu Institute of Technology, Vinayaka Missions Research Foundation Paiyanoor, Chennai, IN
2 Department of Botany, Bharathiar University, Coimbatore, Tamilnadu, IN
3 Department of Plant Science, Bharathidasan University, Tiruchirappalli-620 024, Tamilnadu, IR

Abstract

We have developed simple and efficient regeneration protocol using plant growth regulators and naturally available seaweed. Efficiency of Sargassum polycystum in promoting the growth of Vigna unguiculata at in vitro condition were studied. Equal sized seeds were inoculated in different concentration of seaweeds, we found that 20% of extract shown 100% germination compared to control. Initially, TDZ and Kin were tested for the multiple shoot induction. Best responded concentration was tested in combination with the seaweed extract Sargassum polycystum. We observed increase in the multiple shoot induction from 10.2 shoots per explant to 14.8 shoots per explant. Seaweed extract induced shoot elongation and ischolar_maining at in vitro condition. So we believe that, seaweed extracts has biostimulant activity, the activity of the seaweed extract is due to presence of more than one plant promoting substances.

Keywords

Seaweed, Plant Growth Regulators, Vigna unguiculata, Sargassum polycystum, Tissue Culture.

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